Composite
bce

Part:BBa_K4795007:Design

Designed by: Zilu Liu   Group: iGEM23_Wuhan-Hubei-China   (2023-10-06)


transcribe endoglucanase, extranect glucanase, β-glucosidase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene sequence of flexible connector (G4S)3 was GGGGSGGGGSGGGGS. A suitable restriction enzyme site was added to the end of the primer to facilitate the fusion of DNA and cloned genes.


Source

The cellulase is derived from three different glycoside hydrolase families: BG from GH1 family, CBH from GH7 family and EG from GH12 family

References